A short post to announce TrackMateR, a new R package to analyse TrackMate XML outputs. CodeInstructions Background TrackMate is a plug-in for ImageJ which ships with Fiji. It’s essential for single particle tracking work, particularly for microscopy movies. For example, tracking the movement of fluorescent vesicles inside cells. A tracking session generates a TrackMate XML file.

Slack is a wonderful tool for communicating and organising work in teams. Many academic groups are using the free version (since the pricing is simply incompatible with how we are funded). Slack has had a 10,000 message limit for free tier workspaces for many years but they recently changed it to a 90 day limit. This means any messages older than 90 days will no longer be visible.

Niche tech tips are the cornerstone of this website, and here is another. How to upgrade an iTunes/Music library whilst maintaining the database. I know everyone streams music these days – hence this is likely a very niche tech tip – but I still maintain a large music database on a server. The data in my library is precious (to me) since it tracks my listening habits over 17 years.

Previously I wrote about latin squares and set a puzzle. We can demonstrate this for an n x n latin square where n = 12 In the above images, the normalised latin square only has 12 different pairs out of a possible 66. The density plot shows the pairings in the lower triangle, grey represents 0. A randomly generated latin square (where all rows and all columns feature 1 to 12 exactly once) is shown where all possible pairs are captured.

A comment from a referee led me to find a method to describe curvature of membranes. This is a quick write-up of our solution. I couldn’t find a solution readily available in Fiji, so I created one using a combination of Fiji for tracing the curvature and IgorPro to do the fitting. If there is any interest I can convert this code into an all-Fiji routine. The code is simple and a gist is available here.

This is a ultra-short post related to sending email via the command line on Linux/Rasperry Pi. On 30 May 2022, Google disabled the handy feature that allowed less secure sign in to Google accounts. This feature was a handy way to authenticate command-line routines that send email.

As a project idea for a young engineer we wanted to build a Raspberry Pi-based Weather Station . Our last attempt at building something – a sound-responsive LED display – was a failure. So it was important to build something really easy to help us get back on track. Here are some notes. We essentially followed this guide from Adafruit which links out to this guide for the CircuitPython part.

I read about aesthetic uses of Latin Squares on John Cook’s site a few months ago. Since I maintain a resource to use colour tables in Igor Pro, I thought it would be fun to use Latin Squares to display colour tables for easy visualisation. Briefly, I wrote some code to generate a 9 x 9 latin square and assigned the values 1 – 9 to a colour table wave. The results were nice.

When preparing images for publication, it is good practice to check how accessible they are for colour blind people. Using a simple bit of code, it is possible to check an image – or a whole figure – in ImageJ for accessibility. For example, Figure 1 from our recent paper. Originally looked like this: Using the script we can see how it appears to people with different types of colour blindness.

I recently finished An Essay on Science and Narcissism by Bruno Lemaitre. I found this book really insightful and thought I would write some notes about it here. The book was published in 2016 although I only just got around to reading it. You can grab a copy for free here.

Generating movie files for publication can be a bit tricky. We have a protocol for making them for microscopy data, which simplifies things. However, we recently got this question from a journal: How can we do this? What information is needed for movie files?