There are perennial topics of discussion on Science Twitter. An example of this is: should figures be at the end of a manuscript are interspersed in the text. These topics tend not to be resolved because there are strong arguments (and personal preferences) on each side. I am not sure whether it is even possible to get people to agree with you using reasoned argument on Twitter.

Back in 2015, I wrote a guide to using Trello for lab organisation. I figured it was time for an update because a few things have changed since then and the topic of using Trello for larger labs came up on Twitter. Note that other kanban-style software is available. Basic Trello setup I set up a lab workspace and invited everyone in the team to it. We have three main boards (described below) that everyone in the workspace can see.

PhD students sometimes get the same bad advice on writing their thesis. I call this advice the Rule of Three . Typically, they get told that their thesis: Will take 3 months to write Should have 3 results chapters Should be 300 pages These bits of advice have one thing in common: they are all wrong. If you have been organised (see below), it should not take 3 months to write a PhD thesis.

If I struggle to find the answer to something, it must be worth a post to help out others. So, here’s where to find the php.ini file on a standard WordPress install on Raspberry Pi. I installed a LAMP stack and WordPress on a Raspberry Pi following these excellent instructions. The aim was to import our lab’s ELN onto a fresh WordPress install.

The Retropie project is a wonderful way to play retro games on a Raspberry Pi (see previous post). A number of handheld units are available to make a convincing retro gaming experience. One of these is the Retroflag GPi case, modelled on Nintendo’s Game Boy. It’s possible to order one of these pre-built and even loaded with games, but where is the fun in that? Here is a quick guide to building one.

I have a long-running project Raspberry Pi project to make time-lapse movies of a countryside view (see previous posts). It’s been running for over two years and the results are great. The camera takes images every ten minutes and by assembling movies with different time intervals, you can see seasons change, trees growing or the sun setting and snow melting. The sky is mesmerising to watch.

The aim of this post is to look at revisions of bioRxiv preprints. I’m interested how long preprint versions exist on bioRxiv. In other words: how long do revisions to preprints take? The data from bioRxiv is a complex dataset with many caveats as I’ll explain further down, but some interesting details do emerge. Consider this a sketch of the dataset rather than an in-depth analysis. I’ll walk you through the code.

This post is an update of a previous analysis on quantixed. We have covered publication lag times – the time it takes for a paper go from submitted (received) to accepted and published – a lot on here. It is possible to look at lag times, in R using data from PubMed. Previously my code performed these calculations using an XML file downloaded from the PubMed website.

As the COVID-19 pandemic continues, different countries are experiencing various restrictions including lockdowns. Some of these restrictions alter our ability to do science: by hindering lab access or taking time away from researchers for homeschooling. So, what impact has the pandemic had on scientific output? One way to look at this – for biology – is to look at newly deposited papers on bioRxiv.

Clathrin is a three-legged protein complex or triskelion that can assemble into lattice-like structures. Inside the cell, this assembly helps to create vesicles: tiny packages of membranes containing proteins and goodies for the cell to use. Incredibly our first view of assembled clathrin was made in the 1960s, with resolution improving steadily since then. The image below shows these improvements.

The scientific response to the COVID-19 pandemic has been astounding. Aside from efforts to generate vaccines, the genomic surveillance of the virus has been truly remarkable. For example, the nextstrain project has sequence many SARS-CoV-2 genomes. In fact, the rapid identification of multiple new strains and mutations by diverse groups of scientists has resulted in a nomenclature crisis.